Du Testing
Several weak antigen D forms are recognized, including what was formerly called antigen Du. The term weak antigen D is used to describe those forms of antigen D where the number of red cell D receptors is reduced. Such weak D cells react less strongly than red cells with normal numbers of D receptors. Monoclonal IgM anti-D serum will detect weak antigen D. In some African and other populations, weak antigen D has been found in up to 10% of people. Requirements Purple top vacutainer blood whole blood Anti-D antisera Coombs reagent Test tubes Centrifuge Microscope Slides Procedure Prepare 5% cell suspension of patient. Place one drop of anti-D serum in a test tube. Add to it one drop of patient’s cell suspension. Incubate the t...